EC Number |
Reference |
---|
3.1.3.12 | - |
649561, 695123, 730747, 749237 |
3.1.3.12 | cloned as an operon and expressed in Escherichia coli M15(pREP4) |
705653 |
3.1.3.12 | cloning of wild-type and mutant tagged enzyme with an N-terminal fusion peptide (MGSSH6SSGRENLYFQGH), recombinant expression in Escherichia coli |
750508 |
3.1.3.12 | commentary |
651481, 651667, 653540 |
3.1.3.12 | construction of homozygous and heterozygous deletion mutants, the homozygous deletion mutants has little or no enzyme activity, the complete inactivation of enzyme results in 50fold hyperaccumulation of trehalose 6-phosphate, thermosensitivity, and rapid death of the cells after a few hours at 44°C |
651481 |
3.1.3.12 | DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) and release into the soluble fraction with a natively folded conformation |
713683 |
3.1.3.12 | expressed in Arabidopsis thaliana |
682472 |
3.1.3.12 | expressed in Escherichia coli |
677954, 692676 |
3.1.3.12 | expressed in Escherichia coli as a His-tagged fusion protein |
687429 |
3.1.3.12 | expressed in Escherichia coli BL21 cells |
679760, 728980 |