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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13- 698694
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13cloning a His-tagged p66 or p51 HIV-1 RT DNA fragment into the pQE-9 vector and expression in Escherichia coli strain BL21(DE3)pLys 714475
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expressed in Escherichia coli BL21(DE3) cells 730522
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expressed in Escherichia coli XL1 Blue cells 730494
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression in Escherichia coli 652277, 707794
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression of His-tagged enzyme 716064
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression of His6-tagged p66/p51 HIV-1 reverse transcriptase p51/p66 52A variant C280S, the p66 subunit also contains the mutations K172A and K173A 715864
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression of RNase H domain from residue Y427 to L560 as fusion protein in Escherichia coli 701216
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression of RNase H, in an N-terminally His-tagged construct designed to encode the p51 subunit joined by a linker to the thumb (T), connection (C), and RNase H (R) domains of p66, in Escherichia coli strain MIC2067(DE3) lacking endogenous RNase HI and HII as soluble protein. The construct G provides sufficient RNase H activity to complement Escherichia coli growth at 42°C 713774
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.13expression of the isolated RNase H domain in Escherichia coli 701113
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