EC Number   |
|---|
    3.1.2.22 | - |
| 3.1.2.22 | baculovirally expressed |
| 3.1.2.22 | construction of a UAS:GFP-Ppt1 transgenic fly line. The pUAST:GFP-Ppt1 vector overexpressed using Act5C-Gal4 |
| 3.1.2.22 | DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene |
| 3.1.2.22 | expressed in COS-1 cells |
| 3.1.2.22 | expressed in COS-7 and HEK-293 cells |
| 3.1.2.22 | expressed in Mus musculus |
| 3.1.2.22 | expression as glutathione S-transferase fusion protein in BHK and CHO cells, localization of the recombinant enzyme out side the lysosomes and endosomes |
| 3.1.2.22 | expression in endothelial cells |
| 3.1.2.22 | expression of enzyme carrying naturally occurring mutations in COS-1 cells |
