EC Number |
---|
3.1.11.2 | - |
3.1.11.2 | expression as a GST-fusion protein in Saccharomyces cerevisiae |
3.1.11.2 | expression in Escherichia coli |
3.1.11.2 | into the Gateway entry vector pENTR3C, for expressing Ape2 proteins in the yeast strain BJ5464, the cDNAs are cloned in fusion with GST using the plasmid pBJ842 |
3.1.11.2 | recombinant expression of wild-type and mutant enzymes |
3.1.11.2 | the enzyme containing the mitochondrial targeting signal of manganese superoxide dismutase is expressed in a human malignant breast epithelial cell line, MDA-MB-231, this expression results in a decrease in cell viability following exposure to oxidative stress |