EC Number   |
|---|
   3.1.1.31 | cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15 |
| 3.1.1.31 | expressed as a His fusion protein in Escherichia coli BL21(DE3) cells |
| 3.1.1.31 | expressed in Escherichia coli BL21 (DE3) cells |
| 3.1.1.31 | expression in Escherichia coli |
| 3.1.1.31 | expression of lactonase domain Pf6PGL in Escherichia coli, low recovery of soluble Pf6PGL |
| 3.1.1.31 | gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII |
| 3.1.1.31 | gene PGL3, presence of the C-terminal SKL motif interfered with chloroplast import of a PGL3_N-full-CFP fusion and with that of a PGL3_long medial-CFP fusion (with free N- and C-terminal ends) exclusively labeling peroxisomes, co-expression of thioredoxin Trxm2 with C-terminally truncated enzyme PGL3_N-short and _N-long versions accumulate in the cytosol of tobacco protoplasts and co-localization with Trxm2 in the cytosol and in the chloroplast stroma, recombinant expression of N-terminally CFP-tagged, green-fluorescent enzyme in Nicotiana benthamiana leaves |
| 3.1.1.31 | PCR product cloned into the corresponding sites of the vector pGEX-4T-1 and then introduced into Escherichia coli. Constructs (35S:DELTANTpgl3-GFP and 35S:pgl3 DELTASKL-GFP) together with the 35S:PGL3-GFP construct transformed into the pgl3 background. Escherichia coli cells expressing GST-PGL3 and GST-pgl3 mutants |
| 3.1.1.31 | pgl is a member of the hex regulon |
| 3.1.1.31 | wild-type or pgl mutant expressed in mice |
