EC Number   |
Reference |
|---|
   2.7.8.17 | alpha2beta2gamma2 and alpha2beta2 GlcNAc-1-phosphotransferase are stably expressed in CHO-K1 cells (transmembrane segments are missing) |
725407 |
| 2.7.8.17 | cloning of a single cDNA and gene that encodes a precursor of the alpha- and beta-subunits of human GlcNAc-phosphotransferase (gene symbol, GNPTAB). Transfection of the alpha/beta-subunits precursor cDNA with or without cotransfection of the regulatory gamma-subunitc DNA results in an increase in GlcNAc-phosphotransferase activity on low molecular weight substrate. This demonstrates that the alpha/beta-subunits precursor cDNA encodes the catalytic domain of the enzyme |
662418 |
| 2.7.8.17 | expressed in 293-T cells |
671199 |
| 2.7.8.17 | expressed in CHO-K1 cells |
674538 |
| 2.7.8.17 | expressed in Escherichia coli |
722362 |
| 2.7.8.17 | expressed in Escherichia coli, COS-7 cells and HeLa cells |
725513 |
| 2.7.8.17 | gamma subunit is over-expressed in COS-7 cells |
725457 |
| 2.7.8.17 | gene GNPTAB encodes the alpha- and beta-subunits of GlcNAc-1-phosphotransferase, low efficiency of GNPTAB mRNA expression due to its large size |
738667 |
| 2.7.8.17 | gene GNPTAB encodes the alpha/beta-subunit of GlcNAc-1-phosphotransferase, recombinant expression of C-terminally V5/His-tagged wild-type and mutant enzymes in HEK293 cells |
738667 |
| 2.7.8.17 | gene GNPTAB, the alphabeta precursors of wild-type, and mutant K4Q or S15Y enzymes with a C-terminal V5-tag are expressed in HEK-293 cells |
739540 |
