EC Number   |
Reference |
|---|
    2.7.8.13 | - |
660721 |
| 2.7.8.13 | amplification of the mraY gene, previously called open reading frame Y (ORF-Y,1,080 bp) at 2 min in the chromosome map |
645338 |
| 2.7.8.13 | cloning of the murE-dd1B region, the cell wall biosynthesis and cell division gene cluster, expressed in Escherichia coli BL21(DE3) |
645342 |
| 2.7.8.13 | expression of wild-type enzyme and mutant enzymes D115N, D116N and D267N as C-terminal hexahistidine fusion proteins |
662009 |
| 2.7.8.13 | gene mraY, cloning in Escherichia coli strain DH5alpha |
737788 |
| 2.7.8.13 | heterologous production in Escherichia coli membranes into styrene-maleic acid-wrapped nanodiscs |
762243 |
| 2.7.8.13 | His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha |
662239 |
| 2.7.8.13 | in medium-copy plasmid pBAD30 for complementation studies in Escherichia coli K-12 mutant strain RY3321 lacking endogenous MraY |
692509 |
| 2.7.8.13 | in pET28b for site-directed mutagenesis and expression with N-terminal hexa-His-tag in Escherichia coli C43(DE3) or functional complementation in BW25113-derived thermosensitive strain (EcoliTsMraY) lacking endogenous MraY |
690996 |
| 2.7.8.13 | mraY gene cloned into pASK-IBA3 vector and overexpressed |
645343 |
