EC Number   |
Reference |
|---|
   2.7.7.85 | cdaA and cdaS genes are cloned into the expression vector pET28a, expression in Escherichia coli |
722811 |
| 2.7.7.85 | expression in Escherichia coli |
723182 |
| 2.7.7.85 | expression of truncated CdaA variants in Escherichia coli |
738669 |
| 2.7.7.85 | gene cdaA, genetic organization of CdaA, CdaR, and GlmM encoding genes |
760936 |
| 2.7.7.85 | gene dacA, recombinant expression of N-terminally GST-tagged truncated enzymes DELTA300cdaA and DELTA300cdaA-Y187A, which lacks the transmembrane domain, in Escherichia coli strain BL21(DE3) |
761490 |
| 2.7.7.85 | gene dacZ, DNA and amino acid sequence determination and analysis, phylogenetic tree, the Haloferax volcanii genome encodes one diadenylate cyclase (DAC). The dacZ gene is constantly expressed at different growth phases in rich and selective media. Recombinant overexpression of gene dacZ in HAloferax volcanii strain H26, A double auxotrophic background strain H98 (DELTApyrE2 DELTAhdrB) is also transformed with the dacZ-complementing plasmid [p-dacZ+ hdrB+] and subsequently subjected to identical unselective growth conditions. In these control experiments, several colonies auxotrophic for thymidine and hypoxanthine can be detected. Recombinant expression of His10-tagged DacZ in Escherichia coli strain Rosetta |
761855 |
| 2.7.7.85 | overexpression of the C-terminal His-tagged protein in Escherichia coli |
722145 |
| 2.7.7.85 | the cdaA gene encoding the most-abundant diadenylate cyclase is located within the conserved cdaA-cdaR-glmM module. The bicistronic cdaAR operon encodes the diadenylate cyclase CdaA and its regulator CdaR. The glmM gene encodes the essential phosphoglucosamine mutase GlmM. The lmo2121 and lmo2117 genes of unknown function surround the cdaAR-glmM module |
760995 |
