EC Number   |
|---|
   2.7.7.84 | expression in transgenic Solanum tuberosum plants using the Agrobacterium tumefaciens transfection system, the transformed plants show increased resistance against potato virus X, virus concentration is much lower in leaves and tubers compared to wild-type plants, the virus concentrations are almost that low in transgenic plants expressing the potato virus X coat protein |
| 2.7.7.84 | expression of tagged isozyme OAS1 in Escherichia coli |
| 2.7.7.84 | functional expression of p42 isoform of OAS1 and the p69 isoform of OAS2 in insect cells |
| 2.7.7.84 | gene LB2-5OAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of myc- and His-tagged enzyme in Escherichhia coli |
| 2.7.7.84 | gene N308_13623, DNA and amino acid sequence determination and analysis, sequence comparisons of avian OASL enzymes, phylogenetic analysis, recombinant expression of EGFP-tagged enzyme in HEK-293FT and BHK-21 cells |
| 2.7.7.84 | gene OAS-L, DNA and amino acid sequence determination and analysis, sequence comparisons of avian OASL enzymes, phylogenetic analysis, recombinant expression of EGFP-tagged enzyme in HEK-293FT and BHK-21 cells |
| 2.7.7.84 | gene OAS1, cloning of paralogues OAS1a-OAS1h, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of EGFP-tagged isozyme paralogue in HEK-293T cell |
| 2.7.7.84 | gene OAS1, recombinant expression of His-tagged OAS1 p42 isoform in Escherichia coli strain BL21(DE3)RIL |
| 2.7.7.84 | gene OAS1, sequence comparisons |
| 2.7.7.84 | gene OAS1, the gene is 25414 base pairs long and consists of eight exons. Of these exons, the first five exons from the 5' end are translated into the core protein while the latter three exons enable alternative splicing into the six known protein isoforms of OAS1. Two of these isoforms are the p42 and p46 variants named due to their theoretical size of about 42 and about 46 kDa, the expression pattern between these two variants is related to one single nucleotide polymorphism (SNP), the SNP rs10774671. The SNP is an A/G variation locates in the splice acceptor site of exon 7 which results in either the A allele expressing the p42 isoform or the G allele expressing the p46 isoform. Recombinant expression of C-terminally EGFP-tagged isozymes in HeLa cells, where the C-terminus of OAS1 p46 and its CaaX motif is in itself not enough for membrane translocation |
