EC Number   |
|---|
    2.7.1.49 | - |
| 2.7.1.49 | cDNA clone pBTH1 encoding bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase established in Escherichia coli DH5alpha, expressed in Escherichia coli NI500 and Salmonella typhimurium DM456 by functional complementation |
| 2.7.1.49 | coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis |
| 2.7.1.49 | Escherichia coli BL21(DE3) (pLysS) transformed with pTRD63 carrying ORF2, single gene thiD/J encodes 2 distinct enzyme activities, HMP kinase and HMP-P kinase, functional complemetation of Escherichia coli NI500, overexpression of ThiD |
| 2.7.1.49 | expressed in Escherichia coli |
| 2.7.1.49 | expressed in Escherichia coli strain BLR (DE3) |
| 2.7.1.49 | expression in Escherichia coli |
| 2.7.1.49 | gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.7.1.49 | gene thiD, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.7.1.49 | overexpression in Escherichia coli |
