    2.6.1.27 | gene TSG1 or FIB, DNA and amino acid sequence determination and analysis, genetic complementation is carried out in which a DNA fragment from the wild-type FAZ1 containing the putative promoter region, the entire ORF, and the 3'-untranslated region of TSG1 is introduced into the tsg1 mutant via Agrobacterium tumefaciens-mediated transformation, all transgenic lines harboring the full-length TSG1 transgene display wild-type phenotype. Expression patterns of TSG1 are determined using quantitative real-time PCR and transgenic plants expressing GUS fusion protein driven by the TSG1 promoter |
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