EC Number   |
Reference |
|---|
    2.5.1.62 | amplified by the polymerase chain reaction and cloned into T7 RNA polymerase-based expression plasmid, heterologous expression of chlG in Escherichia coli |
639800 |
| 2.5.1.62 | expression in Escherichia coli |
639803 |
| 2.5.1.62 | expression in Synechocystis sp. PCC 6803 |
759194 |
| 2.5.1.62 | gene chlG, functional recombinant expression of codon-optimizes Synechocystis sp. gene chlG with Synechocystis sp. gene chlP in Rhodobacter sphaeroides lacking genes bchlG and bchlP, mutant subcloning in Escherichia coli strains JM109 and S17-1 |
737331 |
| 2.5.1.62 | gene chlG, recombinant expression of N-terminally 3xFlag-tagged Chl synthase in an CHS-enzyme-deficient Synechocystis sp. strain |
737772 |
| 2.5.1.62 | gene chlG, recombinant expression of N-terminally 3xFlag-tagged Chl synthase in an CHS-enzyme-deficient Synechocystis sp. strain. The cellular level of FLAG-ChlG is lower than of the native ChlG but apparently sufficient to provide chlorophyll for maintaining normal levels of photosynthetic complexes |
739294 |
| 2.5.1.62 | gene chlG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), functional recombinant expression of mutant I44F in Rhodobacter sphaeroides photosynthesis-deficient mutant strain BG1 that lacks a functional bchG gene encoding for bacteriochlorophyll synthase, EC 2.5.1.133 |
738867 |
| 2.5.1.62 | hairpin structure is cloned into Binary plasmid, plants are transformed via Agrobacterium tumefaciens |
710282 |
| 2.5.1.62 | PCR-amplification, in T-vector, expression plasmid pRSET-A in Escherichia coli BL21(DE3)/pLysS |
708935 |
| 2.5.1.62 | subcloning in the pGEM-T vector, cloning in the pBirAR vector for overexpression or gene silencing, transformation of Nicotiana tabacum leaf discs mediated by Agrobacterium tumefaciens strain GV2260 |
710291 |
