EC Number   |
Reference |
|---|
   2.4.2.38 | 5-kb PCR product cloned into the TOPO pCR2.1 vector and maintained in Escherichia coli DH5alpha |
693051 |
| 2.4.2.38 | amplification of the gene with flanking regions (1.3 kb upstream of the start codon, 1 kb downstream of the gene) from serotype D (strain JEC21) genomic DNA for cloning in pCR2.1TOPO, generation of replacement cassettes for replacing endogenous CXT1p: (i) nourseothricin acetyltransferase resistance gene flanked by the CXT1p flanking regions, transformed as linear DNA in CAP67 strain, (ii) cxt1 gene lacking 3 two-thirds of the encoding sequence flanked by its flanking regions, transformed as linear DNA in JEC21 strain |
693051 |
| 2.4.2.38 | DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization |
660169 |
| 2.4.2.38 | DNA and amino acid sequence determination and analysis, gene is located on chromosome 8, functional expression of the enzyme in Pichia pastoris as soluble protein, secretion to the cell culture medium |
660200 |
| 2.4.2.38 | DNA and amino acid sequence determination and analysis, genetic organization and structure, functional expression in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system |
676375 |
| 2.4.2.38 | expressed in Arabidopsis thaliana gux1/2/3 triple mutant and HEK-293F cells |
759938 |
| 2.4.2.38 | expressed in Escherichia coli DH5alpha cells |
730079 |
| 2.4.2.38 | expressed in Nicotiana tabacum leaf epidermal cells |
737023 |
| 2.4.2.38 | expression in insect cells |
394592 |
| 2.4.2.38 | expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium |
658226 |
