EC Number |
Reference |
---|
2.4.1.7 | cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli |
658325 |
2.4.1.7 | DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B |
680980 |
2.4.1.7 | DNA and amino acid sequence determination and analysis, phylogenetic tree |
704230 |
2.4.1.7 | expressed in Acetobacter strain G7, enhanced cellulose production in transformed cells |
637853 |
2.4.1.7 | expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized |
758280 |
2.4.1.7 | expression in Escherichia coli |
679550, 685688 |
2.4.1.7 | expression in Escherichia coli BL-21 Star on large scale |
756927 |
2.4.1.7 | expression in Escherichia coli BL21 |
757282 |
2.4.1.7 | expression in Escherichia coli BL21. Expression conditions are optimized. The highest expression efficiency is obtained at an initial cell density of OD600 0.5, with 0.05 mM isopropyl-beta-D-thiogalactoside, followed by shaking at 180 rpm and incubation at 30°C for 15 h |
758286 |
2.4.1.7 | expression in Escherichia coli strain JM109 |
703795 |