EC Number   |
Reference |
|---|
  2.4.1.292 | gene pglH, cj1129,par of the pgl gene cluster |
720384 |
| 2.4.1.292 | gene pglH, expression of the N-terminally octahistidine-tagged enzyme containing a TEV cleavage sequence in Escherichia coli strain BL-21 RIL using a modified auto-induction method |
718851 |
| 2.4.1.292 | gene pglH, overexpression of His-tagged PglH in Escherichia coli |
720888 |
| 2.4.1.292 | gene pglH, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold (DE3) |
759837 |
| 2.4.1.292 | gene pglH2, genotyping, overview. The variable presence of two open reading frames (ORFs) in the pgl locus includes a putative glycosyltransferase gene, pglG, in addition to a glucosyltransferase-expressing gene, pglH. One subcluster has pglH while the other has the pglH2 variant allele. Strains lacking these two ORFs retain the first 40 bp of pglG and the last 100 bp of pglH. Homologous recombination within the pgl loci through genomic analysis of 100 African serogroup A isolates is detected representing the clonal replacement of hypervirulent meningococcal clone sequence type 7 (ST-7) by the ST-2859 descendant clone. Polymorphisms exist at the gene level described for pglH and pglH2, where only one nonsynonymous mutation is accountable for the glycoform switch from Glc to GlcNAc. One subcluster has pglH while the other has the pglH2 variant allele. The pglH locus shows polymorphisms between STs along the whole gene. A 5' point mutation (guanosine to cytosine) adjacent to the polycytosine [poly(C)] tract is found in individuals 20, 22, and 33 carrying ST-192 isolates |
759430 |
