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EC Number Cloned (Commentary) Reference
Show all pathways known for 2.4.1.277Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.277expression of desVII asaC-terminally His6-tagged protein in Escherichia coli. When affinity-tagged DesVII and DesVIII proteins are coproduced in Escherichia coli, they form a tight (alphabeta)3 complex that is at least 1000-fold more active than DesVII alone. The formation of the DesVII/DesVIII complex requires coexpression of both genes in vivo and cannot be fully achieved by mixing the individual protein components in vitro 714218
Show all pathways known for 2.4.1.277Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.277the recombinant DesVII protein is overproduced in Escherichia coli as a C-terminal His6-tagged fusion protein. The DesVIII protein is produced in Escherichia coli as a fusion with maltose-binding protein at its N-terminus. The fusion protein is cleaved by treatment with protease and untagged DesVIII protein is used in the assays together with DesVII 713756
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