EC Number   |
Reference |
|---|
   2.4.1.143 | - |
658858 |
| 2.4.1.143 | a transformed insect cell line (Tn5beta4GalT) constitutively expresses a mammalian beta-1,4-galactosyltransferase and human beta-1,2-N-acetylglucosaminyltransferase II under the control of an immediate-early promoter. The baculovirus-host system can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans |
658836 |
| 2.4.1.143 | construction of a Gnt II-expressing alg3 mutant strain of Pichia pastoris. Engineering of an artificial glycosylation pathway in Pichia pastoris blocked in dolichol oligosaccharide assembly |
658850 |
| 2.4.1.143 | construction of a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system, and functional expression of His-FLAG-tagged human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region. The enzyme is secreted. The enzyme expressed in silkworm is glycosylated, the structure of N-glycans in the recombinant hGnTII is suggested to be of the complex type, pauci-mannosidic-type glycans, and the removal of the glycans does not affect the enzymatic activity |
759522 |
| 2.4.1.143 | DNA and amino acid sequence determination and analysis, TbGT15 is present as a single copy per haploid genome, recombinant overexpression of full-length TbGT15 with a C-terminal 3x HA epitope tag, semi-quantitative RT-PCR enzyme expression analysis |
759473 |
| 2.4.1.143 | expressed in Sf9 insect cells |
760507 |
| 2.4.1.143 | expressed in Spodoptera frugiperda and Trichoplusia ni cells by using a baculovirus expression vector |
688325 |
| 2.4.1.143 | expression as fusion protein vHis-GlcNAc-TII in Spodoptera frugiperda Sf-9 cells |
644876 |
| 2.4.1.143 | expression in Escherichia coli |
644883 |
| 2.4.1.143 | expression in Spodoptera frugiperda Sf-9 cells |
644873 |
