EC Number |
---|
2.3.1.45 | - |
2.3.1.45 | cloning of cDNAs from melanoma genetic library that induce 9-O-acetylation of sialic acids, expression in COS cells |
2.3.1.45 | expressed in A-549, HEK-293, MDCK-NBL2, MDCK type I, and MDCK type II cells |
2.3.1.45 | expressed in COS-7 cells, GFP-fusion protein expressed in Sf9 cells |
2.3.1.45 | expressed in Escherichia coli AD202 cells |
2.3.1.45 | gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD |
2.3.1.45 | gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD, functional complementation of an enzyme-deficient neuD-mutant Streptococcus sp. strain |
2.3.1.45 | orf11 gene, DNA and amino acid sequence determination and analysis, expression as soluble enzyme in Escherichia coli strain AD202 |
2.3.1.45 | recombinant expression of N-terminally V5-tagged and C-terminally Myc-tagged enzyme in the murine fibroblast cell line LM-TK-, the enzyme co-localizes with the Golgi marker alpha-mannosidase II, and cytosolic and luminal orientation of N- and C-terminus, respectively, recombinant expression of a soluble secreted form of CASD1, which encompasses the SGNH-like luminal domain and a C-terminal Myc-His6-tag, in Spodoptera frugiperda SF9 cells via baculovirus transfection |