EC Number   |
|---|
   2.3.1.175 | - |
| 2.3.1.175 | constructions starting at each of the three in-frame ATG codons of the Acremonium chrysogenum cefG gene (Met1, Met46 and Met60) are expressed in Escherichia coli, obtaining proteins of 49000 Da, 44000 Da and 43000 Da, respectively. All three proteins show deacetylcephalosporin C acetyltransferase activity, separately or in combination |
| 2.3.1.175 | expression in Penicillium chrysogenum |
| 2.3.1.175 | expression in yeast, the leader peptide is not essential for expression of the enzyme activity |
| 2.3.1.175 | expression of the cefG gene from the promoters of 1. the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans, 2. the glucoamylase gene of Aspergillus niger, 3. the glutamate dehydrogenase and 4. the isopenicillin N synthase genes of Penicillium chrysogenum, leads to very high steady-state levels of cefG transcript and to increased deacetylcephalosporin-C acetyltransferase protein concentration |
| 2.3.1.175 | into the pTWIN vector for expression in Escherichia coli BL21DE3 cells |
| 2.3.1.175 | Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene |
