EC Number |
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2.3.1.151 | construction of cDNA library, DNA and amino acid sequence determination and analysis, functional overexpression of wild-type enzyme and expression of mutant enzymes in Escherichia coli strain BL21(DE3) as GST fusion protein |
2.3.1.151 | DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of C-terminally His-tagged BPS in Escherichia coli |
2.3.1.151 | expressed in Escherichia coli |
2.3.1.151 | two cDNAs are isolated and heterologously expressed but both enzymes are inactive |
2.3.1.151 | wild-type benzophenone synthase and enzyme mutants are heterologously expressed as N-terminally His6-tagged proteins in Escherichia coli |