EC Number   |
|---|
   2.3.1.135 | - |
| 2.3.1.135 | DNA sequence determination and analysis of the full length gene including the 3'- and 5'-ends, genetic organization analysis, at least 2 splicing variants, the second of which lacks a 103 nt polynucleotide in the 5'-UTR of the full length transcript |
| 2.3.1.135 | expression in HEK-293 cells |
| 2.3.1.135 | expression in HEK-293T cells |
| 2.3.1.135 | expression in NIH-3T3 cell |
| 2.3.1.135 | expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents |
| 2.3.1.135 | expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) |
| 2.3.1.135 | expression of wild-type and/or mutant enzymes inducibly in HEK-293 cells, transiently in COS-7 cells, and in Escherichia coli strain BL21(DE3) cells |
| 2.3.1.135 | gene lrat is localized on chromosome 4 at locus 4q31.2 |
| 2.3.1.135 | gene Lrat, vector cloning for construction of knockout mutants |
