EC Number   |
Reference |
|---|
   2.1.1.35 | cloning and sequencing of the TRM2 gene encoding enzyme, gene is previously identified as RNC1/NUD1 gene, but does not encode the yNucR endo-exonuclease, TRM2 gene is not essential for cell viability and contains alternative translational start sites, glutathione S-transferase tagged recombinant, Trm2p, is expressed in Escherichia coli |
441380 |
| 2.1.1.35 | enzyme is encoded by the nonessential TRM2 gene |
441381 |
| 2.1.1.35 | expressed in Escherichia coli as a His-tagged fusion protein |
721031 |
| 2.1.1.35 | genes PAB0719 and PAB0760, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) |
705768 |
| 2.1.1.35 | hybrid plasmid carrying trmA gene encoding enzyme is used to amplify enzyme production, trmA gene is sequenced |
441365 |
| 2.1.1.35 | into the pET28b expression vector |
689809 |
| 2.1.1.35 | the plasmids pGP100, pDM4, pKD4 and pKD46 are used for the construction of the mutants |
689248 |
| 2.1.1.35 | trmA gene encodes enzyme, gene is essential for the organism |
441361, 441372, 441376 |
| 2.1.1.35 | trmA gene encoding for RUMT is cloned into a pET vector and transformed into Escherichia coli BL21 (DE3) cells, extremely high-level expression system |
441373 |
| 2.1.1.35 | trmA gene is cloned under control of the inducible lac promoter, DH5alpha |
441378 |
