EC Number   |
Reference |
|---|
   2.1.1.319 | - |
734693, 757532 |
| 2.1.1.319 | expressed in BL21-CodonPlus (DE3)-RIL cells |
756015 |
| 2.1.1.319 | molecular cloning of alternative splice variants of PRMT1 with the use of 3' RACE coupled with next-generation sequencing technology. 58 PRMT1 transcripts are identified, 34 of which are mRNAs encoding protein isoforms, whereas the rest 24 transcripts are candidates for nonsense-mediated mRNA decay |
756861 |
| 2.1.1.319 | prmt6 is cloned in the pnEA-vHis vector between the NdeI and XhoI restriction sites and used to transform Escherichia coli BL21 (DE3) pRARE2 cells. The resulting recombinant protein harbors an amino-terminal hexahistidine tag followed by a Tobacco etch virus (TEV) protease cleavage site. The protein also contains a F315L point mutation, reported for the entry as a natural variant |
757509 |
| 2.1.1.319 | the enzyme (PRMT2) is expressed in insect cells using the baculovirus system |
756703 |
