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EC Number Cloned (Commentary) Reference
Display the reaction diagram Show all sequences 2.1.1.313plasmid pSH26 carrying N terminal 6xHis-Smt3–tagged Bmt5, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37°C to an OD600 of 0.6 730508
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