2.1.1.143 | genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants |
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