EC Number   |
|---|
   1.97.1.4 | - |
| 1.97.1.4 | clone encodes a putative protein of 8532 amino acids |
| 1.97.1.4 | gene pflA, coexpression of pyruvate formate lyase (PFL, gene pflB, UniProt ID P09373 ) with the pyruvate formate lyase activating enzyme, as well as with appropriate electron donors flavodoxin and ferredoxin (encoded by genes fldA and fdx, respectively), in a non-ethanol-producing Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc- MTH1-DELTAT ura3-52), plasmid maps, overview. Reduced flavodoxin is the preferred electron donor for PFL, but coexpression of either of the electron donors has a positive effect on growth under aerobic conditions. Subcloning in Escherichia coli strain DH5alpha. The PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors |
| 1.97.1.4 | gene pflA, ligands CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, CcpA and GlnR bind to promoters containing cre sites |
| 1.97.1.4 | gene pflA, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.97.1.4 | over-expressed in Escherichia coli |
| 1.97.1.4 | overexpression in Escherichia coli |
