EC Number |
---|
1.7.2.3 | - |
1.7.2.3 | amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein |
1.7.2.3 | expressed in Escherichia coli |
1.7.2.3 | expression in strain BL21(DE3) as His-tagged protein |
1.7.2.3 | fusion protein with beta-galactosidase |
1.7.2.3 | gene torA, the torCAD operon encodes the Tor system induced in the presence of trimethylamine oxide mainly during exponential phase |
1.7.2.3 | gene torD, DNA sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as His-tagged protein |
1.7.2.3 | GFP-tagged enzyme expression, the genome sequence of Vibrio fischeri reveals three putative TMAO reductase operons, transcriptional regulation, overview |
1.7.2.3 | overexpression |
1.7.2.3 | overexpression as His-tagged protein |