EC Number   |
|---|
  1.5.99.B4 | genes bll7191, bll7190, and bll7192, sequence comparisons and phylogenetic analysis, recombinant expression from vectors pETDuet-1, pACYCDuet-1, and pCOLADuet-1 with the (His)6-tag attached to the N-terminus of the bll7190 gene, whereas the S-tag is attached to the C-termini of the bll7191 and bll7192 genes, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.5.99.B4 | PpOdhB, PpOdhC, and PpOdhA genes in the PpOdhABC gene cluster, the genes encoding the beta-, gamma-, and alpha-subunits are arranged in tandem on the bacterial genome, genetic structure overview, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression as N-terminally His6-tagged proteins in Escherichia coli strain BL21(DE3), recombinant expression of the PpOdhABC gene cluster in Pseudomonas putida |
| 1.5.99.B4 | the gene cluster from Bradyrhizobium japonicum consists of genes OdhB1-C-A-B2, from which two proteins, OdhAB1C and OdhAB2C, appear through the assembly of each beta-subunit together with common alpha- and gamma-subunits, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression as N-terminally His6-tagged proteins in Escherichia coli strain BL21(DE3), recombinant expression of the PpOdhABC gene cluster in Pseudomonas putida |
