EC Number |
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1.5.3.17 | AtPAO2, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method |
1.5.3.17 | AtPAO3, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, recombinant expression as maltose-binding protein fusion protein in Escherichia coli, and expression of GFP-tagged AtPAO3 in transgenic Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method resulting in increased putrescine levels after feeding of exogenous spermidine |
1.5.3.17 | AtPAO3, expression as GFP-tagged enzyme in plant cells, expression of AtPAO3 fused to the maltose-binding protein in Escherichia coli strain BL21 in a temperature-dependent manner, overview |
1.5.3.17 | AtPAO4, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method |
1.5.3.17 | DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes |
1.5.3.17 | expression in Escherichia coli |
1.5.3.17 | gene AtPAO2, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants |
1.5.3.17 | gene AtPAO3, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants |
1.5.3.17 | gene AtPAO5, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants |
1.5.3.17 | production of recombinant SelPAO5 protein in Escherichia coli |