EC Number |
---|
1.4.3.11 | expressed in Escherichia coli BL21(DE3) cells |
1.4.3.11 | expressed in Pichia pastoris GS115 cells |
1.4.3.11 | expression in Escherichia coli |
1.4.3.11 | expression in Escherichia coli strain JM109 |
1.4.3.11 | expression in Escherichia coli. The recombinant enzyme exhibits low thermostability compared to the enzyme isolated from Streptomyces sp. X-119-6 and is an aggregated form. Proteolysis of the recombinant enzyme with metalloendopeptidase from Streptomyces griseus improves the catalytic efficiency at various pH-values and stabilizes the structure of the enzyme |
1.4.3.11 | mutant enzymes are expressed in Escherichia coli JM109 cells |
1.4.3.11 | overexpression in Escherichia coli JM109 |
1.4.3.11 | successfully expressed by using pHBM905BDM in Pichia pastoris |