EC Number   |
|---|
    1.4.1.9 | - |
| 1.4.1.9 | a series of bifunctional enzyme complexes are produced by fusing leucine dehydrogenase and formate dehydrogenase with different peptide linkers, which are expressed in Escherichia coli, purified, and exhibit varied parental enzyme activities and varied L-tert leucine catalytic efficiency. The enzymatic reaction system for L-tert-leucine production and cofactor regeneration with suitable peptide linker is potentially more excellent than free enzymes with lower labor-cost on purification, better thermal stability and higher catalytic efficiency compared with the free coupling of parental enzymes |
| 1.4.1.9 | chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase |
| 1.4.1.9 | cloned into Escherichia coli JM 109 with a vector plasmid pUC18 |
| 1.4.1.9 | expressed in Escherichia coli Rosetta 2(DE3)pLysS cells |
| 1.4.1.9 | expression in Escherichia coli |
| 1.4.1.9 | expression in Escherichia coli C600 |
| 1.4.1.9 | overexpressed in Escherichia coli |
| 1.4.1.9 | production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale using the runaway replication system Escherichia coli[pIET98] |
| 1.4.1.9 | recombinant expression in Escherichia coli strain SC16591 |
