EC Number   |
|---|
   1.3.7.6 | gene pcyX, phylogenetic analysis and tree, recombinant overexpression of synthetic construct of GST-tagged enzyme |
| 1.3.7.6 | gene pcyX, phylogenetic analysis and tree, recombinant overexpression of synthetic constructs with the original sequences of the genes derived from metagenomic data (termed PcyX-EBQ, PcyX-ECK and PcyX-actino) in Escherichia coli. PcyX-EBQ and PcyX-ECK are functional FDBRs catalysing the reduction of BV via DHBV to PEB, recombinant expression of GST-tagged enzymes in Escherichia coli strain BL21(DE3) |
| 1.3.7.6 | gene pebAB, overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin. Colonies of Synechococcus sp. strain PCC 7002 transformed with the pebAB overexpression cassette mostly exhibit a reddish-brown phenotype |
| 1.3.7.6 | gene pebS, production of His-tagged phycocyanin alpha-subunit-(3Z)-phycoerythrobilin using the alternative FDBR, phycoerythrobilin synthase, PebS, overview. Recombinant functional expression of PebS in Escherichia coli, co-expression with phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, cyanobacterial heme oxygenase, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview |
| 1.3.7.6 | gene pebS, recombinant expression in Escherichia coli strain BL21 (DE3) from plasmid pTDho1pebS, Phycoerythrobilin production in batch-operated bioreactors, phycoerythrobilin enrichment in the bioreactor foam |
