   1.3.3.11 | gene pqqC, enzymes that biosynthesize PQQ are encoded by the pqqABCDE gene cluster and the tldD gene. The tufB promoter is ligated withpqqA, pqqB, pqqC, pqqD, pqqE and tldD, and the pqqA promoter is ligated with pqqB, pqqC, pqqD and pqqE, respectively, through fusionPCR, subcloning in Escherichia coli. Overexpression Gluconobacter oxydans strain WSH-003 of pqqA, pqqB, pqqC, pqqD and pqqE with the pqqA promoter enhances the extracellular PQQ concentration by 38.5%, 68.4%, 19.9%, 30.3% and 8.2%, respectively, whereas production is enhanced by 59.4%, 85.7%, 30.9%, 42.2%, and 19.3%, respectively, using the tufB promoter. Metabolic effects, overview |
