EC Number   |
|---|
   1.3.1.124 | - |
| 1.3.1.124 | creation of a DECR-deficient mouse line, fasted Decr-/- mice display increased serum acylcarnitines, especially decadienoylcarnitine, a product of the incomplete oxidation of linoleic acid (C18:2), urinary excretion of unsaturated dicarboxylic acids, and hepatic steatosis, wherein unsaturated fatty acids accumulate in liver triacylglycerols, metabolically challenged Decr-/- mice turn on ketogenesis, but unexpectedly develop hypoglycemia |
| 1.3.1.124 | expressed in Escherichia coli |
| 1.3.1.124 | expression in Escherichia coli |
| 1.3.1.124 | expression of a highly active truncated soluble protein in Escherichia coli |
| 1.3.1.124 | overexpression of His-tagged truncated wild-type and of mutant enzymes in Escherichia coli strain BL21(DE3), expression as soluble protein, except for mutant D117A |
| 1.3.1.124 | overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of selenomethionine enzyme in Escherichia coli strain B834(DE3) |
