EC Number |
Reference |
---|
1.2.4.4 | - |
349040, 349083, 349084, 349085, 674648, 675563 |
1.2.4.4 | region containing the genes for the entire enzyme complex |
349047, 349048 |
1.2.4.4 | expression in Pseudomonas putida and Escherichia coli |
349048 |
1.2.4.4 | beta-subunit |
349049, 349050, 349089 |
1.2.4.4 | expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu |
349082 |
1.2.4.4 | expression in Escherichia coli |
349086, 762852 |
1.2.4.4 | expression of C-terminally His-tagged lipoic acid-bearing domain of enzyme complex component E2 with an extra methionine at the N-terminus and a glutamic acid and leucine at the C-terminus in Escherichia coli strain BL21(DE3) |
655560 |
1.2.4.4 | expression of the lipoic acid-bearing domain of complex component E2 in Escherichia coli strain BL21(DE3) as His-tagged protein |
656045 |
1.2.4.4 | expression of C-terminally His-tagged lipoic acid-bearing domain LBD, amino acid residues 1-84 of E2b |
656171 |
1.2.4.4 | expression of N-and C-terminally His6-tagged component E1b and mutants, and of C-terminally His-tagged lipoic acid-bearing domain, amino acid residues 1-84 of component E2b, in vitro lipoylation of the latter |
656230 |