EC Number   |
|---|
   1.2.1.46 | construction of a recombinant yeast clone originated from the recipient strain of thermotolerant methylotrophic yeast Hansenula polymorpha which is a NAD+- and glutathione dependent FdDH over-producer |
| 1.2.1.46 | expressed in Escherichia coli |
| 1.2.1.46 | expressed in Escherichia coli BL21(DE3) cells |
| 1.2.1.46 | expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL cells |
| 1.2.1.46 | FLD1 gene with its own promoter is recloned into LEU2-containing integrative plasmid pYT1 devoid of the autonomously replicating sequence to be used for multicopy integration of the gene into chromosome of the recipient strain NCYC 495 |
| 1.2.1.46 | FLD1 gene with the own promoter inserted into the integrative plasmid pYT1 containing LEU2 gene of Saccharomyces cerevisiae (as a selective marker). The constructed vector is used for multi-copy integration of the target gene into genome by transformation of the recipient cells of the strain NCYC 495 |
| 1.2.1.46 | for expression in Escherichia coli BL21 cells |
| 1.2.1.46 | gene fdhA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2 |
| 1.2.1.46 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2. 50% of the recombinant proteins are localized in the supernatant fraction of cell-free extract, suggesting that coexpression with chaperones GroES, GroEL, and Tig has significantly improved the solubilization of Pseudomonas aeruginosa FDH in Escherichia coli |
