EC Number   |
|---|
    1.2.1.20 | expressed in Escherichia coli BW25113(DE3) cells |
| 1.2.1.20 | gene davD, recombinant expression in Corynebacterium glutamicum strain KCTC 1857, coexpression with 5-aminovalerate transaminase (davT, EC 2.6.1.48) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida |
| 1.2.1.20 | gene davD, recombinant expression in Escherichia coli strain W3110, coexpression with Pseudomonas putida gene gabT, encoding 5-aminovalerate aminotransferase, and davA and davB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively. The recombinant Escherichia coli WL3110 strain expressingthe davAB and gabTD genes is cultured in a medium containing D-glucose, L-lysine, and 2-oxoglutarate, and produces glutarate |
| 1.2.1.20 | into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3) |
