EC Number   |
|---|
   1.14.99.63 | agrobacterium-mediated transformation of Haematococcus lacustris |
| 1.14.99.63 | astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight |
| 1.14.99.63 | astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution |
| 1.14.99.63 | expressed in Escherichia coli |
| 1.14.99.63 | expression in an Escherichia coli strain able to accumulate beta-carotene |
| 1.14.99.63 | expression in Escherichia coli |
| 1.14.99.63 | expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX |
| 1.14.99.63 | expression in Escherichia coli, plasmid pBAD24 |
| 1.14.99.63 | expression in Escherichia coli, plasmid pBAD24. Combined overexpression of |
| 1.14.99.63 | expression in Eschreichia coli |
