EC Number   |
Reference |
|---|
   1.14.19.13 | expressed in Saccharomyces cerevisiae InvSc1 cells |
734158 |
| 1.14.19.13 | expressed in Yarrowia lipolytica |
733186 |
| 1.14.19.13 | gene CsFAD3, variant FAD3-1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.13 | gene CsFAD3, variant FAD3-2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.13 | gene CsFAD3, variant FAD3-3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.13 | gene EhDES15, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenic analysis, a codon-optimized EhDES15 gene lacking the 74 amino acid peptide serving as putative bipartite signal and transit peptides at the N-terminus is synthesized and expressed heterologously Synechocystis sp. PCC 6803 cells under control of the trc promoter leading to increased level of n-3 fatty acid species,which are produced at only low levels in wild-type cyanobacterial cells grown at 30°C, semi-quantitative PCR expression analysis. Expression of gene EhDES15 in a Synechocystis mutant lacking the desB gene causes accumulation of 18:3n-3 and 18:4n-3 in the transformed cells |
744387 |
| 1.14.19.13 | gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis |
746264 |
| 1.14.19.13 | gene FAD3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis |
746264 |
| 1.14.19.13 | gene FAD3A, quantitative RT-PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae, CSFAD3A produces 18:2DELTA9,15 from endogenous 18:1DELTA9 when expressed in Saccharomyces cerevisiae |
746012 |
| 1.14.19.13 | gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae |
744576 |
