EC Number |
Reference |
---|
1.14.18.4 | encoded enzyme has both hydroxylase and desaturase activities |
438917 |
1.14.18.4 | expression in Arabidopsis thaliana |
660300 |
1.14.18.4 | expression in Pichia pastoris |
734438 |
1.14.18.4 | expression in Schizosaccharomyces pombe |
733184, 733188 |
1.14.18.4 | FAH gene, unrooted phylogenetic analysis, functional expression in Saccharomyces cerevisiae, expression in transgenic Arabidopsis thaliana plants under control of a seed-specific promoter |
689616 |
1.14.18.4 | fatty acyl desaturase homolog |
438916 |
1.14.18.4 | gene FAH12, recombinant overexpression in Schizosaccharomyces pombe strain ARC010-1, RT-PCR enzyme expression analysis. The enzyme product is toxic for the cells. Gene plg7, encoding phospholipase A2, acts as a multicopy suppressor restoring the growth defect by removing ricinoleic acid from phospholipids and inducing secretion of a part of the released free ricinoleic acid into culture media |
744145 |
1.14.18.4 | gene FAH12, subcloning in Escherichia coli strain DH5alpha, expression in Saccharomyces cerevisiae results in the synthesis of ricinoleic acid and very low levels of di-unsaturated fatty acids. Expression of LlinFAH12 in Arabidopsis thaliana seeds results in hydroxy fatty acid accumulation, fatty acid composition in transformed compared to wild-type plants, overview |
689659 |
1.14.18.4 | gene RcFAH12, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain W303-1A, fatty acid analysis |
746157 |
1.14.18.4 | PCR-amplification, plant transformation by heat shock transformation of Agrobacterium tumefaciens (GV3101) with vector, expression in EMS mutant or T-DNA insertion lines of Arabidopsis thaliana deficient in the production of the enzymes diacylglycerol acyltransferase-1 (DGAT1), phospholipid: diacylglycerol acyltransferase-1 (PDAT1) and the homologue of PDAT1 phospholipid: diacylglycerol acyltransferase-2 (PDAT2) |
697014 |