EC Number   |
|---|
    1.14.15.6 | - |
| 1.14.15.6 | both full-length and an N-terminally truncated form amplified and inserted into TOPO TA PCR cloning vector and subsequently subcloned into the EcoRI restriction site of pCMV-tag2B vector to obtain CYP11A1 proteins with an N-terminal Flag tag or into pEGFP-C2 vector to yield an N-terminal GFP tag |
| 1.14.15.6 | DNA and amino acid sequence determination and analysis, expression analysis and sequence comparisons |
| 1.14.15.6 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, and expression in COS-1 cells with adrenodoxin resulting in significantly more pregnenolone, co-expression with the steroidogenic acute regulatory protein and a human P450scc/adrenodoxin reductase/adrenodoxin fusion constructs. COS-1 cells transfected with a modified construct in which human P450scc is replaced by Dasyatis sabina P450scc show higher rates than cells transfected with Dasyatis sabina P450scc alone. Suncloning in Escherichia coli strain DH5alpha |
| 1.14.15.6 | DNA sequence determination, quantitative enzyme expression analysis in brain prior and after treatment with 4-nonylphenol |
| 1.14.15.6 | expressed from pCMV-SPORT6 vector |
| 1.14.15.6 | expression in Escherichia coli and Saccharomyces cerevisiae |
| 1.14.15.6 | expression in the ventral tegmental area or nucleus accumbens of rat |
| 1.14.15.6 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.14.15.6 | gene CYP11A1, DNA and amino acid sequence determination and analysis, expression pattern analysis, functional expression in COS-7 cells |
