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Cloned (Commentary)
Reference
1.14.11.8
expressed in COS-1 cells
675029
1.14.11.8
expressed in Escherichia coli BL21-AI cells
764104
1.14.11.8
gene TMLH, recombinant overexpression of active and stable TMLH as a His-tagged maltose-binding protein fusion in Escherichia coli strain BL21-AI. Coexpression of the enzyme with chaperones GroES/EL increases the expression level by one order of magnitude
746366
1.14.11.8
recombinant expression of the enzyme fused to MBP
744681
1.14.11.8
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
652164
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