EC Number   |
|---|
   1.13.12.19 | DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae |
| 1.13.12.19 | expressed in Saccharomyces cerevisae. Different cultivation factors on ethylene formation in Saccharomyces cerevisiae expressing the EFE in continuous cultures are investigated. Main finding is that oxygen availability is crucial for ethylene production. By employing three different nitrogen sources it is shown that the nitrogen source available can both improve and impair the ethylene productivity. N-Source/yield ethylene (microgram/g glucose): (NH4)2SO4/164, glutamate/233, glutamate+arginine/96.8 |
| 1.13.12.19 | expression in Escherichia coli |
| 1.13.12.19 | expression in Nicotiana tabacum |
| 1.13.12.19 | gene efe, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree |
| 1.13.12.19 | gene efe, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, heterologous expression of the single efe gene from Pseudomonas syringae results in ethylene production in a number of hosts including Escherichia coli, Saccharomyces cerevisiae, Pseudomonas putida, Trichoderma viride, Trichoderma reesei, tobacco, and cyanobacteria. Methods overview |
| 1.13.12.19 | gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation |
| 1.13.12.19 | gene efe, recombinant enzyme expression in Synechocystis sp. strain PCC 6803, stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3) as the ribosome-binding site, at the slr0168 neutral site. Increase in ethylene production is achieved twofold by RBS screening, improvement of ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-hostself-replicating plasmid, which has a higher copy number than the genome |
| 1.13.12.19 | gene efe, recombinant expression in Escherichia coli strain MG1655, importance of promoter strength on the expression of enzyme EFE in Escherichia coli with stronger promoters producing elevated levels of ethylene, e.g. the Amaranthus hybridus chloroplast psbA promoter (PpsbA), expression method optimization, overview |
| 1.13.12.19 | gene efe, recombinant expression of N-terminally His-tagged or N-terminally His-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3) |
