EC Number |
---|
1.13.11.20 | - |
1.13.11.20 | amplification by PCR and cloning into TA cloning vector, sequencing |
1.13.11.20 | cloned in plasmid pVP16 to produce the enzyme in Escherichia coli B834 pRARE2, an N-terminal fusion to a His-8-maltose binding protein |
1.13.11.20 | cloned into pGEX-6p-1 expression vector, expressed in Escherichia coli Bl21(DE3), glutathione S-transferase-fused CDO is purified and GTS tag is removed by cleavage with PreScission Protease |
1.13.11.20 | cloning of the gene and comparison of the gene to the known rat and human genes, sequencing, profiling of the enzyme mRNA and protein levels in mouse tissues |
1.13.11.20 | expressed as a His-tag fusion protein in Escherichia coli BL21(DE3)pLysS |
1.13.11.20 | expressed as His-tag fusion protein in Escherichia coli |
1.13.11.20 | expressed in BL21(D3)pLysS cells containing the pET-14b/CDO-ORF plasmid |
1.13.11.20 | expressed in BL21(DE3) cells |
1.13.11.20 | expressed in Escherichia coli |