EC Number |
---|
1.1.98.2 | - |
1.1.98.2 | expressed in Escherichia coli C41(DE3) cells |
1.1.98.2 | expressed in Escherichia coli Top10 cells |
1.1.98.2 | expression in Escherichia coli BL21(DE3), no significant homology with NADP+-dependent glucose-6-phosphate dehydrogenases |
1.1.98.2 | gene Rv0132c, sequence comparisons and homology modeling of Rv0132c, expression of Rv0132c-D38 construct lacking the predicted N-terminal signal sequence in Mycobacterium smegmatis by nickel affinity chromatography and gel filtration, co-expression with coenzyme F420 |
1.1.98.2 | initial attempts to produce recombinant FGD1 using Escherichia coli as a host is unsuccessful, but when the alternative host Mycobacterium smegmatis is used, soluble protein yields of 7 mg/l of culture are achieved |
1.1.98.2 | recombinant expression of His-tagged wild-type and selenomethionine-labeled FGD1 in Mycobacterium smegmatis strain mc24517 |