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Results 1 - 7 of 7
EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2-
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2expressed in Escherichia coli C41(DE3) cells
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2expressed in Escherichia coli Top10 cells
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2expression in Escherichia coli BL21(DE3), no significant homology with NADP+-dependent glucose-6-phosphate dehydrogenases
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2gene Rv0132c, sequence comparisons and homology modeling of Rv0132c, expression of Rv0132c-D38 construct lacking the predicted N-terminal signal sequence in Mycobacterium smegmatis by nickel affinity chromatography and gel filtration, co-expression with coenzyme F420
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2initial attempts to produce recombinant FGD1 using Escherichia coli as a host is unsuccessful, but when the alternative host Mycobacterium smegmatis is used, soluble protein yields of 7 mg/l of culture are achieved
Display the word mapDisplay the reaction diagram Show all sequences 1.1.98.2recombinant expression of His-tagged wild-type and selenomethionine-labeled FGD1 in Mycobacterium smegmatis strain mc24517
Results 1 - 7 of 7