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Results 1 - 8 of 8
EC Number
Commentary
Reference
erxpression in Escherichia coli
expression in Escherichia coli
expression of the gene encoding for the isoform AlgE6 R2 module, residues 534-693, in Escherichia coli
gene algE4, recombinant expression in Escherichia coli strain ER2566, and expression of chimeric enzyme mutant AlgE64; gene algE6, recombinant expression in Escherichia coli strain ER2566, and expression of chimeric enzyme mutant AlgE64, recombinant expression of the R-modules, AlgE6R1, AlgE6R2, and AlgE6R3
gene c5epi, DNA and amino acid sequence determination and analysis revealing eight partial amino acid sequences, SjC5-I to -VIII, highest frequency of clone SjC5-VI is found and elucidated with respect to full-length cDNA and putative gene structure, sequence comparisons, RT-PCR enzyme expression analysis in brown algae, functional heterologous expression of His-tagged MC5E clone SjC5-VI in Spodoptera frugiperda Sf9 cells via baculovirus transfection system, and protein secretion
gene MEP13, genetic structure, phylogenetic tree, recombinant expression of the codon-optimized His-tagged catalytic domain, MEP13-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL in inclusion bodies. The usage of Escherichia coli strain Rosettagami2(DE3)pLysS produces larger and more significant inclusion bodies than Escherichia coli strain BL21CodonPlus(DE3)RIPL; gene MEP18, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP18-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP21, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP21-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP25, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP25-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP26, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP26-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP27, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP27-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP28, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP28-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP29, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP29-C5, in Escherichia coli strain BL 21CodonPlus(DE3)RIPL; gene MEP2, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP2-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP4, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP4-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP6, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP6-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL; gene MEP7, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP7-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL
recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha; recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha; recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Results 1 - 8 of 8