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EC Number
; Neurospora crassa
; Saccharomyces cerevisiae gene CYC3
cloning in Escherichia coli
cloning of the gene CaCYC3 in Escherichia coli
coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
expression of the enzyme in Escherichia coli cytosplasm, coexpression with several mutant variants of holocytochrome c552 from Hydrogenobacter thermophilus, the latter are expressed in the periplasm, or several cytochrome c variants from Equus caballus
heterologous expression in a multicopy yeast vector
recombinant coexpression of GST-tagged wild-type and mutant enzymes with cytochrome c in Escherichia coli strain RK103 membranes
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