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EC Number
expressed in Corynebacterium glutamicum ATCC 13032
expressed in Escherichia coli BL21 (DE3) pLysS cells
expression in Escherichia coli
gene cadA from strain V02-64, DNA and amino acid sequence, and gene cluster and intergenic region determination and analysis, subcloning in Escherichia coli, quantitative analysis of lysine decarboxylase expression levels dependent on acid conditions at pH 4.0-7.5, primer extension analysis, the gene encoding the enzyme is located in an acid-induced operon, overview, cadA mRNA exhibited about 40fold greater expression at pH 5.0 than at pH 5.5, in cells exposed to pH 7.5, the cadA transcript shows the largest quantity under all conditions, the amount of cadA single transcript is nearly 100fold higher than the polycistronic cadBA transcript for each pH condition
gene cadA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cadA, encoded in the cadBA operon, also coding for a lysine-cadaverine antiporter, CadB, expression of cadA in Escherichia coli strain S17-1 and S17-1lambdapir, transcription of cadBA in a pH-dependent manner, overview
gene cadA, recombinant enzyme expression in Escherichia coli strain XL-1 Blue
gene cadA, recombinant expression of His-tagged wild-type and T88 mutants in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene cadA, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
Results 1 - 10 of 22 > >>