EC Number |
Reference |
---|
3.4.23.38 | - |
648179 |
3.4.23.38 | expression and partial characterization of soluble recombinant PM I from Plasmodium falciparum in which a truncated form of PM I (Lys77P-Leu329) (P indicates a propart residues) is fused to thioredoxin in the pET32b(1) vector, Trx-tPM I and expressed in Escherichia coli Rosetta-gami B (DE3)pLysS. The soluble fusion protein is purified from cell culture using a combination of Ni21 affinity and gel filtration chromatography and is capable of autocatalytic activation at pH 4.05.5, which occurrs at Leu116P-Ser117P, seven residues upstream of the native cleavage site (Gly123P-Asn1) |
682637 |
3.4.23.38 | expression in Escherichia coli |
648189, 718040 |
3.4.23.38 | expression of proplasmepsin I and V110P-proplasmepsin in Escherichia coli |
648190 |
3.4.23.38 | expression of truncated proPfPM1 K110pN mutant in Escherichia coli |
696329 |
3.4.23.38 | initial attempts to express full-length proplasmepsin I gene in Escherichia coli results in very low expression levels, possibly due to the toxic effects of the hydrophobic segment, and no activation to mature plasmepsin I is ever observed. Recombinant proplasmepsin I is expressed from pETPMI, a construct that is prepared from pET3a and contains the last 48 residues of the propart followed by the 328 residues of mature plasmepsin I. It is necessary to introduce a processing site, V110P, into the zymogen that renders the precursor capable of undergoing autoactivation |
648190 |