Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Cloned(Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 6 of 6
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38- 648179
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38expression and partial characterization of soluble recombinant PM I from Plasmodium falciparum in which a truncated form of PM I (Lys77P-Leu329) (P indicates a propart residues) is fused to thioredoxin in the pET32b(1) vector, Trx-tPM I and expressed in Escherichia coli Rosetta-gami B (DE3)pLysS. The soluble fusion protein is purified from cell culture using a combination of Ni21 affinity and gel filtration chromatography and is capable of autocatalytic activation at pH 4.0–5.5, which occurrs at Leu116P-Ser117P, seven residues upstream of the native cleavage site (Gly123P-Asn1) 682637
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38expression in Escherichia coli 648189, 718040
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38expression of proplasmepsin I and V110P-proplasmepsin in Escherichia coli 648190
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38expression of truncated proPfPM1 K110pN mutant in Escherichia coli 696329
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.38initial attempts to express full-length proplasmepsin I gene in Escherichia coli results in very low expression levels, possibly due to the toxic effects of the hydrophobic segment, and no activation to mature plasmepsin I is ever observed. Recombinant proplasmepsin I is expressed from pETPMI, a construct that is prepared from pET3a and contains the last 48 residues of the propart followed by the 328 residues of mature plasmepsin I. It is necessary to introduce a processing site, V110P, into the zymogen that renders the precursor capable of undergoing autoactivation 648190
Results 1 - 6 of 6