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EC Number
Cloned (Commentary)
cloned into plasmids under T7 promoter control and cotransfected into HeLa-MHVR cells infected with recombinant vaccinia virus
expressed in Escherichia coli
expressed in Escherichia coli Rosetta (DE3)pLysS cells
expression in Escherichia coli
expression in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-binding protein fusion protein. With both overexpression systems the recombinant PLP-1 exhibits trans cleavage activity. When PLP-1 is expressed as a polypeptide that includes additional viral sequences at the carboxyl terminus of the predicted PLP-1 domain, a fivefold increase in proteolytic activity is observed
Results 1 - 6 of 6